. Author manuscript; available in PMC: 2013 Jan 24.

Published in final edited form as: ACS Nano. 2012 Jan 3;6(1):696–704. doi: 10.1021/nn204165v

Table 2.

Cellular uptake of selected aptamers by different cell lines.

Cell lines	Cell Source	XEO2	XEO2mini	XEO6	XEO6mini	XEO9
PC3	Prostate carcinoma (androgen- independent)	++++	++++	+	+	++++
LNCaP	Prostate carcinoma (androgen-dependent)	++++	+	++++	++++	++++
RWPE-1	Prostate normal epithelial	++	----	----	----	++
BPH	Prostate benign hyperplastic epithelial	++	+	----	----	++
HeLa	Cervical carcinoma	----	----	----	----	----
SKBR3	Breast carcinoma	+	+	+	+	+
A375	Melanoma	----	----	----	----	----
U373MG	Brain glioblastoma-astrocytoma	----	----	----	----	----
T98G	Brain glioblastoma	----	----	----	----	----
U87MG	Brain glioblastoma-astrocytoma	----	----	----	----	----
A549	Lung carcinoma	----	----	----	----	----
SKOV3	Ovary adenocarcinoma	----	----	----	----	----

Note: The internalization capacity of selected aptamers in different cell lines was evaluated by R value as the following threshold. The mean fluorescence of selected sequence (MF sequence) in the FACS analysis was normalized to the mean fluorescence of initial library (MF lib) in the same experiment condition. R= (MF sequence −MF lib) / MF lib.

^----

R < 0.5;

⁺

0.5≤R≤1;

⁺⁺

1 < R < 1.5;

⁺⁺⁺

1.5 ≤ R < 2;

⁺⁺⁺⁺

R > 2.