Table 1.

Oligonucleotide primers used for the cDNA cloning of zebrafish COMT and for the quantitative real-time RT-PCR analysis of the developmental stage-dependent expression of the zebrafish COMT

Target sequence		Sense and antisense oligonucleotide primers used
I. For cDNA cloning*:
COMT	Sense:	5’ -CGCGGATCCATGCTGTGGGTTGTGTTGGCAGTGGTGGTG-3’
(Full-length)	Antisense:	5’ -CGCGGATCCTCATCCTAAGAAGACCGATTTCTCCAGGCC-3’
COMT	Sense:	5’ -CGCGGATCCCACGATGTGGTCCATCAGCGGCTCCTGAAC-3’
(Truncated)	Antisense:	5’ -CGCGGATCCTCATCCTAAGAAGACCGATTTCTCCAGGCC-3’
II. For real-time RT-PCR analysis**:
COMT	Sense:	5’ -TCACGACCACAGCGCATCT-3’
	Antisense:	5’ -CCCACATTCATGGCCCATT-3’
β-Actin	Sense:	5’ -CGAGCTGTCTTCCCATCCA-3’
	Antisense:	5’ -TCACCAACGTAGCTGTCTTTCTG-3’

^*Recognition sites of BamHI restriction endonuclease in the oligonucleotides are underlined. Initiation and termination codons for translation are in bold type.

^**The sense and antisense oligonucleotide primer sets listed were verified by BLAST Search to be specific for the zebrafish COMT or β-actin nucleotide sequence.