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. Author manuscript; available in PMC: 2013 Dec 5.
Published in final edited form as: J Am Chem Soc. 2012 Nov 20;134(48):19746–19757. doi: 10.1021/ja308346b

Figure 10.

Figure 10

FTIR spectra of solutions of 1.1 mM iron(II)-azurin in 25 mM MOPS buffer at pH 10, with addition of 5 mM (purple) and 88 mM (red) cyanide. For comparison, the spectra of iron(II) azurin (green) and cyanide (blue dashed) are overlaid on the spectrum. The peak at 2080 cm-1 is due to free cyanide. The peak at 2043 cm-1 at low [CN–] fits to the sum of two Lorentzian peaks (Figure S9), consistent with a superposition of the residual protein peak at 2048 cm-1 and the C-N stretching band of the cyanide adduct at 2039 cm-1.