Table 1.

Sustained action of substoichiometric amounts of an oligonucleotide in directing RNA cleavage by Hs-RNase H1.

See Materials and Methods and Results & Discussion for experimental procedures. The turnover number (column 3) is defined as the number of rounds of RNA cleavage in 30 minutes, and is determined by multiplying the RNA:DNA ratio (column 1) by the fraction of RNA cleaved (column 2).

RNA:DNA ratio	Fraction of heteroduplex cleaved in 30 min.	Turnover Number
10	0.90	9
50	0.56	28
100	0.29	29