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Kinetic parameters of Hs-RNase H1 and the H264A mutant.
See Materials and Methods, and Results & Discussion for the determination of the kinetic values and best-fit error estimates. Reported values are from reactions performed at 30°C.
| kcat (sec−1) | Km (nM) | kcat/Km (M−1 sec−1) | Vmax (pM sec−1) | |
|---|---|---|---|---|
| Hs-RNase H1 | 3.5 ± 0.5 | 97 ± 21 | 3.6 × 107 | 35 ± 5 |
| H264A | 0.025 ± 0.004 | 106 ± 34 | 2.4 × 105 | 0.25 ± 0.04 |
