Figure 5.

The effect of chloramphenicol on the kinetic characteristics of substrate-dependent O₂ evolution (▪) and HCO₃⁻ transport (▴) after a 4-min fast-induction period. High-Ci cells from Synechococcus sp. strain PCC7002 (10 μg of Chl mL⁻¹) were allowed to run out of Ci for 4 min in the presence of CA (100 units mL⁻¹) and chloramphenicol (200 μg mL⁻¹). During the subsequent dark period of 2 to 3 min, the cells were diluted to a [Chl] of 2.5 μg mL⁻¹ with fresh CO₂-free medium containing the same amount of chloramphenicol. AZA (50 μg mL⁻¹) was added to inhibit the remaining CA activity.