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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Nat Struct Mol Biol. 2012 Oct 28;19(12):1316–1323. doi: 10.1038/nsmb.2403

Figure 2. ctRio2 has ATPase activity in vitro.

Figure 2

The amount of released free phosphate (Pi) (a and c) or phosphorylated protein (b) was analyzed in single-turnover experiments using 1μM of the indicated purified recombinant protein. (a) and (c) ATP and Pi were separated by thin-layer chromatography. (b) the amount of phosphorylated protein was analyzed by SDS-PAGE. Dried gels and TLC plates were exposed overnight on a phosphorimager screen (see experimental procedures for a full description). Note that 100 times more of the reactions were loaded in panel b in comparison to panel a and c.