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. 2012 Sep 11;22(12):1681–1695. doi: 10.1038/cr.2012.132

Figure 4.

Figure 4

Stn1 deficiency delayed G-overhang lengthening in S phase and compromised G-overhang shortening in G2 phase. (A) FACS analysis of DNA content in synchronized HeLa cells expressing shLuc and shStn1. (B) G-overhang measurement of shLuc and shStn1 at 7 h (late S/G2) and 12 h (next G1) after releasing from doublethymidine block using the overhang protection assay. (C) Quantitation of mean overhang lengths. Two-tailed t-test was used to calculate statistical significance. Results were from at least 4 independent experiments. Error bars: S.E.M. (D) Non-denaturing in-gel hybridization of ss G-rich DNA with C-probe during the cell cycle. (E) Quantitation of (D). Results were from 3 independent experiments. Error bars: S.D.