Figure 5.

Stn1 unlikely plays a role in restricting end resection at C-strand. (A) G-overhang measurement of leading and lagging telomeres from HeLa cells using the overhang protection assay. (B) Quantitation of mean overhang lengths from A. Results were from 3 independent experiments. Error bars: S.E.M. (C) In-gel hybridization of separated leading and lagging telomeres from BJ/E6/E7 cells with C-rich probe. (D) Quantitation of the abundance of ss G-rich DNA from C. Signals were normalized to the total telomere signals. Results were from 4 independent experiments. Error bars: S.E.M. Two-tailed t-test was used in all the above experiments to calculate statistical significance.