Figure 1.

Expression patterns of CBL2 and CBL3 in Arabidopsis. (A) RT-PCR analysis of CBL2 and CBL3 transcripts during seedling emergence and in different organs of Arabidopsis plants. Total RNA was isolated from germinating seeds and young seedlings (1 day, 2 days, 3 days, 7 days, 21 days after sowing) or from various tissues (root, leaf, stem, flower, silique) of wild-type Col-0 plants. RT-PCR was performed with CBL2/3-specific primers or ACTIN2-specific primers at indicated numbers of PCR cycles. Genomic DNA was used as a control template to rule out its possible contamination in each cDNA sample. PCR products were visualized by agarose gel electrophoresis followed by ethidium bromide staining. (B) CBL2 and CBL3 promoter-GUS expression in transgenic Arabidopsis plants. Histochemical GUS staining was carried out at different germinating stages and in various tissues of CBL2 (the first and third rows) or CBL3(the second and fourth rows) promoter-GUS transgenic plants. (a) and (f) one-day old germinating seed, scale bar = 0.5 mm; (b) and (g) two-day old germinating seed, scale bar = 0.5 mm; (c) and (h) three-day old seedling, scale bar = 2 mm; (d) and (i) seven-day old seedling, scale bar = 2 mm; (e) and (j) cotyledon from a five-day old seeding, scale bar = 1 mm; (k) and (p) young root, scale bar = 1 mm; (l) and (q) rosette leaf, scale bar = 5 mm; (m) and (r) section of an inflorescence stem, scale bar = 0.05 mm; (n) and (s) flower, scale bar = 0.5 mm; (o) and (t) silique, scale bar = 0.5 mm.