Figure 6.

Ion sensitivity of the cbl2 cbl3 double mutant. (A-G) Growth of wild-type Col-0 and cbl2 cbl3 mutant plants under various ionic stress conditions. Five-day old Col-0 and cbl2 cbl3 seedlings were transferred onto 1/6 MS medium (A) or 1/6 MS medium supplemented with 60 mM NaCl (B), 60 mM KCl (C), 40 mM CaCl₂ (D), 100 μM ZnCl₂ (E), 50 μM FeCl₃ (F) or 20 μM CuCl₂ (G), respectively. Pictures were taken on the 21th day after transfer. (H) Measurements of seedling fresh weight of Col-0 and cbl2 cbl3 on the 21th day after transfer. Error bars represent SE of 12 independent seedlings from triplicate experiments. Asterisks indicate statistically significant difference between wild-type Col-0 and cbl2 cbl3 double mutant (Student's t-test, ^*P < 0.05).