Altered ion profiles in the cbl2 cbl3 double mutant. (A) Comparison of the root ionome of wild-type Col-0, cbl2 cbl3 and vha-a2 vha-a3 mutants. (B) Comparison of the leaf ionome of wild-type Col-0, cbl2 cbl3 and vha-a2 vha-a3 mutants. Root and leaf samples from wild-type Col-0 and cbl2 cbl3 mutant plants hydroponically grown under the same condition were analyzed for an array of ion contents using inductively coupled plasma-mass spectrometry. Ion profile data among Col-0, cbl2 cbl3 and vha-a2 vha-a3 plants were compared. For each element, the mean concentration in the wild-type Col-0 was used as the control, and log2 of the ratio of each mutant divided by the control was calculated and shown in the diagrams. Data represent mean ± SE of 12 independent plant samples in three replicate experiments.