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Primers used for RT-PCRanalysis
| Primers | Product (bp) | Cycles | |
|---|---|---|---|
| Osteocalcin |
184 |
30 |
|
| forward |
5’-GGGCAATAAGGTAGTGAACAG-3’ |
|
|
| reverse |
5’-GCAGCACAGGTCCTAAATAGT-3’ |
|
|
| Drg-1 |
433 |
31 |
|
| forward |
5’-CGCTGAGGTGAAGCCTCTGGTG-3’ |
|
|
| reverse |
5’-AGGGTTGTTGAGTGCGAAGCGG-3’ |
|
|
| β-actin |
275 |
22 |
|
| forward |
5’-CAGGAGATGGCCACTGCCGCA-3’ |
|
|
| reverse | 5’-AGGGTTGTTGAGTGCGAAGCGG-3’ | ||
