Figure 2. Chondrogenic and osteogenic differentiation of ATDC5 cell by BMP-2 polyhedra.

BMP-2 polyhedra or CP-H polyhedra were spotted and ATDC5 cells were seeded on and around polyhedron spot. Early phase differentiation of cartilage nodule formation was examined with an amount of glycosaminoglycan by alcian blue staining (A and B). At late phase osteogenic differentiation of ATDC5, mineralization of extracellular matrix is measured by arizarin red staining (C and D).