Figure 1. Relationship between short-circuit current (I_sc) and HCO₃⁻ secretion.

Forskolin (10 μmol l⁻¹) and GlyH-101 (100 μmol l⁻¹) were added to control Calu-3 cells expressing mutated shRNA. Note the close agreement between I_sc and HCO₃⁻ secretion, and their sensitivity to the CFTR channel inhibitor GlyH-101 (n= 4). •, I_sc, short-circuit current; º, HCO₃⁻ secretion as measured using pH-stat.