Table 2.

Composition of fluid secreted by Calu-3 monolayers

	Control transfected	CFTR knock-down	Initial medium
Measured [Cl−] (mmol l−1)	120 ± 5	123 ± 6	135 ± 16
Measured pH	7.55 ± 0.04	7.28 ± 0.02	7.43 ± 0.03
Calculated [HCO3−] (mmol l−1)	31.35 ± 2.1	16.08 ± 0.52***	24.4 ± 2.2
Osmotic pressure (mosmol l−1)	309 ± 16	301 ± 6	310 ± 16

Fluid was collected daily during forskolin (10 μmol l⁻¹) stimulation and pooled for analysis. Values are mean ± SEM, n= 3–12 each condition.

^***P < 0.001 comparing CFTR knock-down vs. control transfected.