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. 2012 Aug 20;590(Pt 21):5335–5348. doi: 10.1113/jphysiol.2012.236885

Figure 7. Cys158 of the Kir5.1 in S-glutathionylation.

Figure 7

A and B, in inside-out patches, the Kir4.1–Kir5.1 (C158T) (A) and Kir4.1–Kir5.1 (C158A) (B) mutations eliminated the responses of the channels to 5.0 mm GSSG. C and D, the mutant Kir4.1–Kir5.1 channels also lost sensitivity to 50 μm DTNP. Note that each panel was made of 8 superimposed traces. Calibration: 100 pA and 50 ms for D, and 200 pA and 50 ms for the rest. E, statistically, the mutant responses to GSSG and DTNP were significantly different from the WT channel (**P < 0.01; ***P < 0.001, n= 6, one-way ANOVA).