Table 2.
Total CPI-17 | Phosphorylated CPI-17 | Contraction | ||||
---|---|---|---|---|---|---|
Concentration (μm) | n | Concentration (μm) | n | % of maximum | n | |
MA | 12.1 ± 1.0* | 13 | 4.1 ± 0.3* | 5 | 80 ± 2* | 12 |
Aorta | 4.7 ± 0.3* | 12 | 0.3 ± 0.1* | 4 | 54 ± 2* | 11 |
Phosphorylation of CPI-17 and contraction were measured at a freezing point 30 s after PE stimulation in both artery types. To calculate the CPI-17 concentration in smooth muscle cells, the protein content of a mammalian cell was assumed to be 18% of the total cell weight (Dimopoulos et al. 2007).
Significant difference (P < 0.05) from that of MA.