Figure 1. Activation of cardiac IGF-2R via Leu²⁷IGF-2 infusion into the coronary artery did not increase cardiomyocyte proliferation.

There was no change in markers of proliferation such as the percentage of Ki67+ mononucleated cardiomyocytes (A) or PCNA (B) and regulators of cell cycle at G₁ phase such as p21 (C) or p27 (D) protein abundance in the left ventricle of control compared with Leu²⁷IGF-2-infused fetuses. Representative blots from 3 animals in each group for each protein. Sample size for each group is indicated in the bar.