Figure 5. Activation of cardiac IGF-2R via Leu²⁷IGF-2 infusion into the coronary artery causes significant changes in the protein expression of markers of cardiac hypertrophy.

The amount of ANP protein (A) was significantly increased in the left ventricle of Leu²⁷IGF-2-infused fetuses, while SERCA (C) and phospho-troponin I (E) were decreased. There were no changes in the amount of ANP (B), SERCA (D) and phospho-troponin I (F) proteins in the right ventricle of hearts from control compared with Leu²⁷IGF-2-infused fetuses. Representative Western blots from 3 animals in each group for each protein. Sample size for each group is indicated in the bar. *Significantly different from control fetuses (P < 0.05).