Fig 1. c-Abl inhibition does not alter L-selectin shedding from neutrophils following activation with IL-8, fMLP, or osmotic swelling under static conditions.

untreated (A-F) and STI571 treated (G-L) neutrophils were stimulated with 1 μL water (B, H), 5 μL DMSO, 1 nM IL-8 (D, J), 5 nM fMLP (E, K), or by osmotic swelling (F, L) and then labeled with anti- L-selectin and CBRM1/5 antibodies. STI571 treated (G) and untreated neutrophils (A) were also labeled with APC- and FITC- conjugated isotype controls. Neutrophils were fixed and analyzed via flow cytometry. STI571 treatment did not have a significant effect on L-selectin shedding (M) and β₂ integrin activation (N) due to exposure to IL-8, fMLP, or osmotic swelling under static conditions. In (M) and (N), all activation conditions were significantly different from the unactivated samples (p<0.05). These results are representative of five separate experiments using cells from different consenting donors. Statistical evaluation of the results were conducted using two-tailed paired t-tests with a significance level of α=0.05 for each type of cell stimulation. Error bars displayed are SEM.