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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Biomaterials. 2012 Sep 17;33(35):9037–9048. doi: 10.1016/j.biomaterials.2012.08.052

Fig. 10.

Fig. 10

Western blot membranes showing the expression of HIF-1α and HIF-1β in spheroid, monolayer (Normoxia) and monolayer cells treated with cobalt chloride (Hypoxia) in BT20 (A and B), MCF7 (C and D) and co-culture spheroids (E and F). The ratio indicated in the figure corresponds to BT20:MCF7:MCF10A cells in co-culture. The band intensity computed using ImageJ indicates that expression of HIF-1α increased in co-culture conditions (G).