Figure 6.

(a) Number of HDFs within formulation F as determined using the CyQuant assay, and provided as relative fluorescence, using initial cell seeding densities of 0.4, 0.8, 1.5, and 3.0 million cells/mL. (b) Metabolic activity per cell for HDFs seeded within formulation F using the different cell seeding densities. Metabolic activity was determined using an MTS assay, providing relative absorbance, which was then divided by the average relative fluorescence from the CyQuant assay. *P < 0.05 compared to all other formulations. N = 6 for each seeding density and each assay at each timepoint; values are mean ± SD. Statistical analysis is within each timepoint.