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Comparative study employing six different secondary reagents for detecting EGFR immunoreactivity with a mouse monoclonal antibody, clone: DAK-H1-WT in a representative case of advanced colon cancer. A. CSA II, B. SSMAX, C. PolyVue, D. Novolink, E. EnVision™ FLEX+ and F. MACH3. CSAII and Novolink give the strongest reactivity, but with cytoplasmic diffusion with CSAII. Membrane reactivity is sharply accentuated with Novolink.
