Table 1.
EGFR immunostaining in various combinations
| Monoclonal antibody to EGFR | Intensity Score | CSA II | SSMAX | PolyVue | Novolink | EnVision™ FLEX+ | MACH3 |
|---|---|---|---|---|---|---|---|
| EGFR 2.5 | 0 | 0 (0%) | 4 (80%) | 1 (20%) | 0 (0%) | 0 (0%) | 1 (20%) |
| 1+ | 2 (40%) | 1 (20%) | 2 (40%) | 0 (0%) | 2 (40%) | 0 (0%) | |
| 2+ | 3 (60%) | 0 (0%) | 2 (40%) | 3 (60%) | 2 (40%) | 3 (60%) | |
| 3+ | 0 (0%) | 0 (0%) | 0 (0%) | 2 (40%) | 1 (20%) | 1 (20%) | |
|
| |||||||
| DAK-H1-WT | 0 | 0 (0%) | 2 (40%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) |
| 1+ | 1 (20%) | 2 (40%) | 0 (0%) | 0 (0%) | 1 (20%) | 1 (20%) | |
| 2+ | 3 (60%) | 1 (20%) | 5 (100%) | 0 (0%) | 3 (60%) | 2 (40%) | |
| 3+ | 1 (20%) | 0 (0%) | 0 (0%) | 5 (100%) | 1 (20%) | 2 (40%) | |
0: negative, 1+: weak intensity, 2+: moderate intensity, 3+: strong intensity.