Table 2.
Characteristics of the Leishmania strains and Bhutanese clinical isolates used for ITS sequence comparison and phylogenetic analysis
| Taxa | WHO-code | Origin | Pathology | ITS sequence type* | GenBank accession no. |
|---|---|---|---|---|---|
| L. infantum | MHOM/TN/80/IPT1 | Tunisia | VL | A | AJ000289 |
| L. donovani | MHOM/CN/00/Wangjie1 | China | VL | C | AJ000294 |
| MCAN/SD/00/LEM3946 | Sudan | CanVL | D | AJ634356 | |
| MHOM/ET/72/GEBRE1 | Ethiopia | VL | E | AJ634367 | |
| MHOM/ET/67/HU3 | Ethiopia | VL | F | AJ634373 | |
| MHOM/KE/85/NLB323 | Kenya | VL | G | AJ000297 | |
| MHOM/IN/00/DEVI | India | VL | H | AJ634376 | |
| MHOM/IN/1983/CHANDIGARH | India | VL | n.a. | AM901449 | |
| Patient 18 (BHUTAN-Trashigang)† | Bhutan | VL | n.a. | JQ730001 | |
| Patient 19 (BHUTAN-Samtse)† | Bhutan | VL | n.a. | JQ730002 |
*
ITS sequences type according to Kuhls and others.13 Type A has been described on Mediterranean, Chinese, and American strains, type E and F in Ethiopian and Sudanese strains, and type G in Keyan and Indian strains; n.a.: not assigned, different to those described by Kuhls and others.
†
Clinical samples, not culture isolates, thus WHO-code is not provided for this two samples.