Skip to main content
. 2012 Sep 21;21(26):5484–5499. doi: 10.1093/hmg/dds393

Figure 8.

Figure 8.

The E421K substitution alters microtubule dynamics. (A) A representative carbendazim sensitivity assay. Diploid cells were serially diluted and plated on rich YPD media containing increasing concentrations of carbendazim, a microtubule destabilizing agent. The Tub2p haploinsufficient cells (TUB2+/−) were super-sensitive to carbendazim. In contrast, both heterozygous tub2-E410K and heterozygous tub2-E421K cells displayed increased resistance to carbendazim compared with WT cells. (B) Chart depicting a summary of more than three independent carbendazim assays with concentration ranging from 0 –21 μg/ml. Shaded boxes depict the degree of carbendazim resistance. (C) Life-time history plots of two representative individual microtubules from diploid WT (left), heterozygous tub2-E410K (center) or heterozygous tub2-E421K (right) cells in G1. (D) Parameters of dynamic instability determined for each strain. Notably, tub2-E421K microtubules show increased rates of polymerization, depolymerization, and reduced time spent in attenuation. Error given as standard deviation. Number of events is in parentheses. MT, microtubule. *P < 0.05, **P < 0.005 versus WT by unpaired Student's t-test.