Figure 2.

siRNA-mediated depletion of DCX and DCLK results in enhanced tangling of growth cone microtubules. (A–C) Immunofluorescence staining of tubulin in three control growth cones. Growth cone microtubules of control neurons typically exhibit a curvy contour, and many can be traced from the central into the peripheral domain. (D–F) Three growth cones of neurons treated with siRNA SMARTpools to DCX and DCLK. Overall the appearance of the microtubule array in the growth cone is notably more tangled than in control growth cones. Scale bar = 6 μm.