Fig. 4.—

Scatterplot showing differentially regulated transcripts from S. flava 5-day-old larvae raised on WT versus GKO plants. Transcripts that were significantly induced by glucosinolates in the diet of S. flava are in red, and those repressed by glucosinolates in the diet are in purple. Two RNA pools (biological replicates) for each treatment were each sequenced on single Illumina lanes (four lanes total). These sequences were mapped back to an assembled transcriptome that was scaffolded on the D. grimshawi genome. A total of 341 transcripts were differentially regulated. Larvae used for this analysis were obtained from those used in the weight-gain analysis presented in figure 3.