Table 1.

Ages of Nodes in Figure 1 and Supplementary Figure S1, Supplementary Material Online, Inferred by r8s Analysis

Node	Calibration Range (MYBP)	Transcriptome Alignment, Age ± SD (MYBP)	Multiple Scaptomyza spp.
			Age ± SD (MYBP)	Bootstrap Support
1	50.5–75.3	74.81 ± 1.14	71.19 ± 4.10
2		62.55 ± 0.74	61.44 ± 3.90	100
3		48.85 ± 0.97	50.72 ± 3.01	100
4	35.3–53.1	35.34 ± 0.73	36.75 ± 1.97	100
5		8.80 ± 1.38	11.40 ± 0.84	100
6		6.48 ± 0.80	8.96 ± 0.78	57
7		4.50 ± 0.70	6.85 ± 0.62	100
8		2.31 ± 0.52	3.25 ± 0.40	100
9	0.56–1.14	1.14 ± 0.05	1.14 ± 0.00	100
10	34.2–51.6	43.26 ± 0.74	41.70 ± 2.92	100
11		32.04 ± 0.66	31.82 ± 2.57	34
12	23.9–37.1	27.06 ± 0.66	29.76 ± 2.25	100
13		–	21.69 ± 1.76	100
14		–	14.70 ± 1.26	100
15		–	6.31 ± 0.63	100

Note.—Node numbers refer to nodes labeled in figure 1 and supplementary figure S1, Supplementary Material online. Date intervals for calibrating are in the second column. Divergence dates are in millions of years before present (MYBP) followed by standard deviation of dates estimated by profiling nodes across 1,000 bootstrapped replicates. Two phylogenies were used: the first used characters from an alignment of nucleotide sequences from the 12 Drosophila genomes and Scaptomyza flava transcriptome, and the second used 9 genes from a broader sampling of drosophilids, including herbivorous and nonherbivorous Scaptomyza species. All bootstrap values for the tree generated for transcriptome alignment were 100.