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. Author manuscript; available in PMC: 2012 Dec 6.
Published in final edited form as: Mol Cell. 2002 Jul;10(1):139–149. doi: 10.1016/s1097-2765(02)00576-2

Figure 1. Na-ATP-Dependent Dimerization of the E171Q Mutant of MJ0796.

Figure 1

Tryptophan fluorescence anisotropy titrations were conducted at 24°C on the Y11W mutant of MJ0796 either with or without the hydrolytically deficient E171Q mutation. The protein was used at a 1 μM concentration. Relative total fluorescence values are shown in the bottom panel, while fluorescence anisotropy values from the same titrations are shown in the top panel. The variables “Kd” and “n” refer to the dissociation constant and the cooperativity, respectively, and were determined by nonlinear curve fitting (solid lines).