Fig. 3.

Proteasomal degradation of bilaterally protected p21 and α-syn. Substrates with two GFP domains flanking either an internal p21 (A) or α-syn (B) domain were analyzed as in Fig. 2. For the degradation time course, 100 nM substrate was incubated with 10 nM 20S or 26S proteasome. For fluorescence monitoring, the amount of proteasome was increased to 20 nM.