Fig. 3.

Flagellar targeting by the NPM motif and flanking sequences. (A) The structure of the N-terminal domain was predicted using Rosetta software (Yarov-Yarovoy et al., 2006). The model shows the NPM motif (red) forming a hook at the tip of the loop connecting two predicted α-helixes (green). (B) The segment of the model that corresponds to amino acids 81–113. The NPM motif (red) is represented here using stick figures. (C) Targeting of LmxGT2 chimera proteins. Yellow arrowheads show examples of overlapping GFP expression and tubulin. White arrowheads indicate absence of GFP signal. The numbers below each image indicate the percentage of cells that target to the pellicular plasma membrane (PPM), the flagellar membrane only (FM) or both membranes (FM+PMM). ‘n’ indicates the total number of cells quantified for each mutant. Not shown is the flagellar-pocket-only localization of GT1(1–130)::GT2::GFP, which is approximately 4%. Scale bar: 5 µm.