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. Author manuscript; available in PMC: 2013 Nov 16.
Published in final edited form as: Immunity. 2012 Nov 1;37(5):930–946. doi: 10.1016/j.immuni.2012.07.016

Figure 1.

Figure 1

Intestinal T cells predominantly express CEACAM1-S isoforms. (A) Cell surface expression of CEACAM1 (CC1 antibody) on CD3+ T cells from spleen, MLN, PP or lamina propria (LP) of colon from C57BL/6 mice. (B) qPCR for transcriptional analysis of CEACAM1-L and -S in CD3+ T cells isolated from spleen, MLN, blood, PP or segments of small intestine and colon LP from WT mice. (C) Cell surface expression of CEACAM1 (5F4 antibody) on CD3+ T cells from human peripheral blood (PBMC) or colon LP. (D) Semi-quantification of the relative transcription of CEACAM1-L and -S in human CD3+ T cells isolated from peripheral blood with indicated treatment or from fresh colon LP. (E) qPCR for quantification of CEACAM1-L and -S transcription in naïve CD3+ T cells from WT mice, MLN T cells from WT mice and CD3+ T cells isolated from spleen and colon LP of Rag2−/− mice adoptively transferred with WT CD4+ naïve T cells. (F) T cells isolated from mouse LP were expanded with ConA and IL-2 in vitro for two weeks and the expression of CEACAM1-L and -S were determined by qPCR.