Skip to main content
. Author manuscript; available in PMC: 2013 Nov 16.
Published in final edited form as: Immunity. 2012 Nov 1;37(5):930–946. doi: 10.1016/j.immuni.2012.07.016

Figure 3.

Figure 3

Overexpression of CEACAM1-4S on T cells enhances the nuclear translocation of NFAT1. (A–B) Immunoblot of whole cell lysates (Total), cytosolic (Cyto) and nuclear (Nuc) extracts of splenic CD4+ T cells isolated from (A) WT and 4S Tg or (B) Ceacam1−/− and Ceacam1−/−-4S Tg (4S Tg/KO) mice with anti-NFAT1, anti-GAPDH or β-actin antibodies. (C) Frozen sections of PP from WT or 4S Tg animals were stained with anti-CD4 (red), anti-NFAT1 (green) and DAPI (blue). Areas of overlap are indicated by white arrows. Data are representative of 3 pairs of animals.