FIG. 4.

(A) A bar graph exhibiting significantly (P<0.05) reduced infarct volume in rats treated with an IA infusion of poststroke MNCs as compared with saline-treated control animals. Prestroke MNCs did not lead to a reduction in infarct volume. Data are mean±SD. N=6 per group.*P<0.05 compared with saline controls. (B) Twenty-four hours after ischemic stroke, Long Evans rats received an intracarotid injection of pre- or poststroke MNCs that were labeled with Q tracker nanocrystals prior to infusion. Labeled cells were quantified in the peri-infarct area at 24 h after IA infusion of MNCs. N=3 per group. Data are mean±SD. (C) Representative fluorescence microscopic images: the upper panel shows Q-dot labeled MNCs (green) in the peri-infarct region at 1 day after IA infusion (under low magnification); the lower panel under high magnification represents the outlined area in the upper panel and shows MNCs containing Q-dot nanocrystals (green). These cells are stained with the nuclear dye, 4′,6-diamidino-2-phenylindole (DAPI, blue). (D) A bar graph exhibiting serum cytokine measurements from animals treated with pre- or poststroke MNCs administered at 24 h after stroke. Cytokines were measured at 3 days after stroke. *P<0.05, compared with sham group; ^#P<0.05, compared with saline treatment; ^&P<0.05, compared with poststroke MNCs. Data are mean±SD. N=6 per group. Color images available online at www.liebertpub.com/scd