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. 2012 Jun 28;21(18):3270–3277. doi: 10.1089/scd.2012.0032

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Copyright 2012, Mary Ann Liebert, Inc.

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FIG. 1. — Long-term assessment of xenogeneic EGFP⁺-ASC engraftment into young rabbit tissues after fetal transplantation. (A) Efficient lentiviral vector-mediated transduction of adipose tissue-derived mesenchymal stem cells (ASCs). Lentiviral vector-transduced ASCs were visualized by enhanced green fluorescent protein (EGFP) fluorescence (magnification: 400×; scale bar=25 μm). (B) Polymerase chain reaction-mediated detection of integrated, EGFP⁺-ASC-derived, pWPT-EGFP provirus into genomic DNA isolated from a representative EGFP⁺-ASC-treated young rabbit at 16 weeks after transplantation. EGFP-specific primers amplified a 417-bp fragment. Lane 1, 1 kb DNA ladder; lanes 2–9 analysis performed in the indicated tissues.