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. 2012 Dec;27(10):701–710. doi: 10.1089/cbr.2012.1245

FIG. 2.

FIG. 2.

GA induces apoptosis of human osteosarcoma cells. (A) Hoechst staining of osteosarcoma cells treated with 25, 50, and 75 μM for 24 hours, respectively. The nuclei were stained by Hoechst 33258 and visualized under fluorescence microscope (magnification, 200×; scale bar=100 μm). (B) The apoptotic ultrastructure of osteosarcoma cells treated by GA. The cells were examined under a transmission electron microscope (×3700 power). In the 50 μM GA group, typical apoptotic cells were observed in U-2OS and MNNG/HOS; scale bar=2 μm. (C) Annexin V-FITC/PI staining of osteosarcoma cells treated by GA. (a) control group; (b) 25 μM GA; (c) 50 μM GA; (d) 75 μM GA. The data were representative of three independent experiments. (D) GA induces the activation of caspase-3 and -9 while does not significantly influence the activation of caspase-8. Lysate was prepared from cells grown in the absence or presence of GA (25, 50, and 75 μM) for 24 hours and tested for activities. Data represented the mean of three measurements±SD. **p<0.01 versus control group; #p<0.001 versus control group.