Table 1.

Primers for Regular Polymerase Chain Reaction and Splicing by Overlap Extension–Polymerase Chain Reaction (5′-3′)

Fragments	Primers
IgGκ	1F: GCT GGA AGC TTA GCA TGG AGA CAG ACA CAC
	1R: GTC AGC ACA ATG TCG TCA CCA GTG GAA CCT
Anti-erbB2 scFv	2F: CTG GTG ACG ACA TTG TGC TGA CCC AAA CT
	2R: TCG GCT GAC GAG ACG GTG ACT GAG GTT
Fc-CD28-CD3ζ	3F: CCG TCT CGT CAG CCG AGC CCA AAT CTC CT
	3R: AAC CAT CGA TCA GCA TCT CTC CAG TAT TAG CG

The underlined sequences are the sites for restriction digest, the gray-highlighted sequences are complimentary tailer sequences, and the dotted sequences are initiation or termination codons, respectively. 1F and 2R were used as primers for amplifying the IgGκ/anti-erbB2 scFv fragment in SOE-PCR. 1F and 3R were used as primers for amplifying the IgGκ/anti-erbB2 scFv/Fc/CD28/CD3ζ fragment in SOE-PCR.

scFv, single-chain variable fragment; SOE-PCR, splicing by overlap extension–polymerase chain reaction.