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Cell attachment densities on rigid substrata are ligand dependent. (A) Density of MDA-MB-435 cells attached to substrates with Young’s moduli of 34 and 0.6 kPa modified with N-cad.Fc (homophilic) and E-cad.Fc (heterophilic) ligands 4 hr after cell seeding in serum-free medium. (B) Density of MDCK cells on substrates with Young’s moduli of 34 and 0.6 kPa coated with E-cad.Fc (homophilic) and N-cad.Fc (heterophilic) ligands, 4 hr after cell seeding in 0.5 v/v% FBS.
