Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Sep 15;125(18):4405–4412. doi: 10.1242/jcs.109603

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012. Published by The Company of Biologists Ltd

PMC Copyright notice

Fig. 5. — OAP imaging in astrocytes. (A) Primary, well-differentiated cultures of mouse astrocytes were fixed and stained with NMO-IgG (red) and C-terminus anti-AQP4 antibody (green) and imaged using TIRFM and two-color dSTORM. (B) dSTORM of astrocytes exposed to control or NMO serum, or treated with 2-bromopalmitate. Average OAP areas summarized at the bottom (mean ± s.e.m., n = 6, P<0.05). (C) BN-PAGE (top) and two-dimensional BN-SDS-PAGE (bottom) of whole-astrocyte homogenates after incubation with control or NMO sera as in B. (D) SDS-PAGE of astrocyte homogenate (H) and plasma membrane fraction (PM) after incubation with control or NMO serum, probed with antibodies against Na⁺/K⁺ ATPase (top) and AQP4 (bottom).