Fig. 6.

OAP clustering as a non-specific response to cytotoxicity. (A) TIRFM of primary cultures of mouse astrocytes incubated with rAb-53, with or without C1q, fixed, and stained with AQP4 (red) and C1q (green) antibodies. Right panels show dSTORM of AQP4, labeled with Alexa-Fluor-488-conjugated C-terminus anti-AQP4 antibody. (B) TIRFM and dSTORM of mouse astrocytes incubated with rAb-53 and 20% fresh human complement or thapsigargin, stained as in right panels of A. (C) OAP areas (mean ± s.e.m., n = 3, P<0.01).