Table 2. Summary statistics of variance in relative fitness.

1° Line	1° Fitness	n_sublines (1°, MA)	VL*,1°	V L*,MA	VM* (×104)	VE*,1°	VE*,MA	$h_M^2$ (×103)
504	Low	10, 22	0.02 (0.04)	0.17 (0.15)	5.25 (5.12)	0.64 (0.15)	0.94 (0.21)	0.73 (0.75)
508	Low	10, 21	0.02 (0.03)	0.01 (0.03)	0.22 (0.88)	0.59 (0.10)	1.18 (0.17)	0.28 (1.08)
547	Low	10, 22	0.08 (0.09)	0.16 (0.11)	3.61 (3.80)	0.80 (0.27)	1.06 (0.22)	0.40 (0.43)
550	Low	10, 22	0	0.02 (0.04)	0.82 (1.20)	0.66 (0.14)	0.79 (0.17)	0.13 (0.20)
579	Low	10, 20	0.12 (0.12)	0.01 (0.03)	0.15 (0.66)	1.67 (0.45)	1.26 (0.21)	0.01 (0.05)
579.2	Low	20,25	0.02 (0.04)	0.21 (0.13)	6.52 (4.60)	1.45 (0.24)	1.73 (0.25)	0.42 (0.29)
	Low ave	10, 21.4	0.04 (0.02)	0.09 (0.03)	2.65 (1.13)	0.85 (0.18)	1.09 (0.12)	0.35 (0.10)
522	High	10, 24	0.06 (0.04)	0.16 (0.06)	3.57 (2.40)	0.24 (0.04)	0.21 (0.04)	1.61 (1.08)
537	High	10, 24	0.00 (0.00)	0.07 (0.04)	2.21 (1.40)	0.19 (0.06)	0.27 (0.05)	0.98 (0.62)
566	High	10,22	0.02 (0.02)	0.09 (0.06)	2.33 (1.90)	0.28 (0.05)	0.35 (0.06)	0.73 (0.58)
583	High	10, 24	0.00 (0.00)	0.10 (0.04)	3.29 (1.30)	0.15 (0.04)	0.26 (0.04)	1.59 (0.62)
587	High	10, 24	0.00 (0.00)	0.14 (0.08)	4.76 (2.60)	0.28 (0.04)	0.39 (0.08)	1.41 (0.73)
	High ave	10, 23.6	0.02 (0.01)	0.11 (0.02)	3.23 (0.46)	0.23 (0.03)	0.30 (0.03)	1.26 (0.17)
—	All lines	10, 22.8	0.04 (0.02)	0.10 (0.02)	2.94 (0.50)	0.54 (0.13)	0.69 (0.15)	0.81 (0.18)

Column headings are: 1° Line, first-order MA line; 1° Fitness, fitness group (high or low) of the 1° MA line; n_sublines, number of sublines (1° control pseudolines, 2° MA) within a 1° MA line; V_L*,1°,among-line variance of 1° control pseudolines; V_M*, mutational variance; V_E*,1°,environmental (within-line) variance of the 1° controls; V_E*,MA, environmental (within-line) variance of the 2° MA sub-lines; $h_M^2$, mutational heritability (V_M*/V_E*); V_E* here is the average of the 1° controls and the 2° MA lines. Line 579.2 is the re-assay of line 579 (see text). Shaded rows are mean values of high and low 1° fitness groups; the contribution of line 579 is the unweighted mean of the two assays. SEMs in parentheses. All variances standardized by the relevant group mean; see Materials and Methods for details of calculations.