Figure 5 .

The Effect of CG8445 RNAi on the expression of Notch signaling targets in the wing imaginal disc. The expression of Wg (magenta; A) and Cut (magenta; C) along the dorsal-ventral boundary (i.e., presumptive wing margin) is activated by Notch signaling in a wild-type (WT) wing disc. Knocking down the expression of Notch receptor by the ptc-Gal4 driver along the anterior-posterior boundary (marked by GFP) led to a complete loss of downstream targets, Cut (E, F) and Wg (G, H), on the presumptive wing margin cells intersecting the dorsal-ventral boundary. In contrast, the expression of both Cut (J) and Wg (L) was unaffected in wing discs ectopically expressing faf RNAi, consistent with previous studies indicating that faf mutant flies did not exhibit any defect in the wing. Knockdown of the expression of CG8445 by RNAi in the wing disc resulted in modulate reduction of Cut (arrow; N) and Wg (arrow; P), suggesting that CG8445 is necessary to positively regulate Notch signaling. All phenotypes are fully penetrant (n > 20 discs).