Figure 6 .

CG9769 positively regulates Notch signaling in the wing disc. When the expression of CG9769 was knocked down by RNAi at the anteroposterior boundary of the wing disc by the dpp-Gal4 driver (marked by GFP; A and C), Notch signaling activity was largely compromised as indicated by decreased expression of Cut (B) and Wg (D). The abundance of Notch protein, as examined by specific antibodies raised against either the extracellular domain (NECD; E and F) or the intracellular domain (NICD; G and H) of the Notch receptor, also was reduced. The effect of CG9769 on the production of Notch protein was cell-autonomous (arrows; J), as demonstrated in FLIPout clones (Ito et al. 1997) overexpressing CG9769 RNAi (positively marked by GFP; I). All phenotypes are fully penetrant (n > 20 discs).