Table 5. Vulval induction pattern in Class 2 and 3 mutants.
% VPC Fate Pattern (F/3°/I) |
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Class | Genotype | VPC Induction Score | P3.p | P4.p | P5.p | P6.p | P7.p | P8.p | n |
AF16 | 3 | 61/39/0 | 0/100/0 | 0/0/100 | 0/0/100 | 0/0/100 | 0/100/0 | 101 | |
2 | unc(sy5505) | 1.7 ± 1.3 | 39/4/0 | 0/35/0 | 0/0/50 | 0/0/75 | 0/0/48 | 2/37/0 | 52 |
sy5505; bhEx141 | 1.2 ± 1.2 | 25/2/00 | 8/8/00 | 0/0/31 | 0/0/62 | 0/0/31 | 4/13/0 | 52 | |
sy5505; bhEx142 | 1.4 ± 1.1 | 24/4/0 | 2/18/0 | 0/0/38 | 0/0/62 | 0/0/38 | 4/11/0 | 45 | |
Cbr-unc-84(sy5506) | 1.9 ± 1.2 | 31/14/0 | 10/18/0 | 0/0/53 | 0/0/82 | 0/0/51 | 14/25/0 | 51 | |
sy5506; bhEx139 | 2.9 ± 0.4a | 54/30/0 | 0/86/0 | 0/0/96 | 0/0/100 | 0/0/94 | 0/93/0 | 91 | |
sy5506; bhEx152 | 2.9 ± 0.4a | 57/31/0 | 2/90/0 | 0/0/94 | 0/0/100 | 0/0/96 | 0/94/0 | 51 | |
3 | lin(bh7) | 2.3 ± 1 | 77/23/0 | 54/46/0 | 28/4/68 | 5/0/95 | 32/2/66 | 43/57/0 | 56 |
lin(bh14) | 2.3 ± 1 | 91/9/0 | 71/29/0 | 35/0/65 | 1/0/99 | 33/0/67 | 65/35/0 | 78 | |
Cbr-lin-39(bh20) | 0.2 ± 0.4 | 100/0/0 | 100/0/0 | 100/0/0 | 85/0/15 | 100/0/0 | 100/0/0 | 155 | |
bh20; bhEx134 | 1 ± 0.9a | 100/0/0 | 97/3/0 | 93/0/7 | 40/0/60 | 71/0/29 | 99/1/0 | 88 | |
bh20; bhEx132 | 0.5 ± 0.5a | 100/0/0 | 100/0/0 | 99/0/1 | 54/0/46 | 100/0/0 | 100/0/0 | 81 | |
Cbr-lin-39(bh23) | 0 | 96/4/0 | 96/4/0 | 88/12/0 | 96/4/0 | 96/4/0 | 100/0/0 | 50 |
VPC fates are classified into three categories: F, fused with hyp7 without division, 3°, fused with hyp7 after one cell division, I, induced (either 1°, 2°, or a hybrid fate that could not be uniquely classified). See Materials and Methods for details. For sy5505 and sy5506 animals missing VPCs were excluded from the analysis. VPC, vulval precursor cells.
VPC induction is significantly higher compared to the parental strain, P < 0.0001.