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. 2012 Dec 1;2(12):1625–1641. doi: 10.1534/g3.112.004598

Table 5. Vulval induction pattern in Class 2 and 3 mutants.

% VPC Fate Pattern (F/3°/I)
Class Genotype VPC Induction Score P3.p P4.p P5.p P6.p P7.p P8.p n
AF16 3 61/39/0 0/100/0 0/0/100 0/0/100 0/0/100 0/100/0 101
2 unc(sy5505) 1.7 ± 1.3 39/4/0 0/35/0 0/0/50 0/0/75 0/0/48 2/37/0 52
sy5505; bhEx141 1.2 ± 1.2 25/2/00 8/8/00 0/0/31 0/0/62 0/0/31 4/13/0 52
sy5505; bhEx142 1.4 ± 1.1 24/4/0 2/18/0 0/0/38 0/0/62 0/0/38 4/11/0 45
Cbr-unc-84(sy5506) 1.9 ± 1.2 31/14/0 10/18/0 0/0/53 0/0/82 0/0/51 14/25/0 51
sy5506; bhEx139 2.9 ± 0.4a 54/30/0 0/86/0 0/0/96 0/0/100 0/0/94 0/93/0 91
sy5506; bhEx152 2.9 ± 0.4a 57/31/0 2/90/0 0/0/94 0/0/100 0/0/96 0/94/0 51
3 lin(bh7) 2.3 ± 1 77/23/0 54/46/0 28/4/68 5/0/95 32/2/66 43/57/0 56
lin(bh14) 2.3 ± 1 91/9/0 71/29/0 35/0/65 1/0/99 33/0/67 65/35/0 78
Cbr-lin-39(bh20) 0.2 ± 0.4 100/0/0 100/0/0 100/0/0 85/0/15 100/0/0 100/0/0 155
bh20; bhEx134 1 ± 0.9a 100/0/0 97/3/0 93/0/7 40/0/60 71/0/29 99/1/0 88
bh20; bhEx132 0.5 ± 0.5a 100/0/0 100/0/0 99/0/1 54/0/46 100/0/0 100/0/0 81
Cbr-lin-39(bh23) 0 96/4/0 96/4/0 88/12/0 96/4/0 96/4/0 100/0/0 50

VPC fates are classified into three categories: F, fused with hyp7 without division, 3°, fused with hyp7 after one cell division, I, induced (either 1°, 2°, or a hybrid fate that could not be uniquely classified). See Materials and Methods for details. For sy5505 and sy5506 animals missing VPCs were excluded from the analysis. VPC, vulval precursor cells.

a

VPC induction is significantly higher compared to the parental strain, P < 0.0001.