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. 2012 Dec 1;2(12):1651–1660. doi: 10.1534/g3.112.004333

Figure 3 .

Figure 3 

Su(z)2 and additional PcG genes are strong suppressors of the dE2f1-dsRNA phenotypes in the wing. (A) Part of L3-L4 intervein region of a control Drosophila wing (ptc-Gal4/+). Ptc-Gal4 is expressed in the L3-L4 intervein region. At 22∼23°, when dE2f1-dsRNA (line #3) is expressed under control of ptc-Gal4, the L3-L4 intervein region is reduced by ∼50%, as shown in (B) (w1118; ptc-Gal4, UAS-dE2f1dsRNA#3/+; +/+). This wing phenotype can be strongly suppressed by Df(2R)Exel6062 (C: w1118; ptc-Gal4, UAS-dE2f1dsRNA#3/Df(2R)Exel6062; +/+), or the Su(z)21.a1 allele (D: w1118; ptc-Gal4, UAS-dE2f1dsRNA#3/Su(z)21.a1; +/+). The modification of the wing phenotype can be quantified by measuring the width of L3-L4 intervein region (E), and the genotypes of data presented in (E) are as follows: (a) w1118; ptc-Gal4/+; +; (b) w1118; ptc-Gal4, UAS-dE2f1dsRNA#3/+; +; (c) w1118; ptc-Gal4, UAS-dE2f1dsRNA#3/Df(2R)Exel6062; +/+; (d) w1118; ptc-Gal4, UAS-dE2f1dsRNA#3/Su(z)21; +/+; (e) w1118; ptc-Gal4, UAS-dE2f1dsRNA#3/Su(z)21.b7; +/+; (f) w1118; ptc-Gal4, UAS-dE2f1dsRNA#3/Su(z)21.a1; +/+; and (g) w1118; ptc-Gal4, UAS-dE2f1dsRNA#3/Su(z)2k06344; +/+; (h) w1118; ptc-Gal4, UAS-dE2f1dsRNA#3/+; Pcf01890/+; (i) w1118; ptc-Gal4, UAS-dE2f1dsRNA#3/+; Pc3/+; (j) w1118; ptc-Gal4, UAS-dE2f1dsRNA#3/+; His2AvD810/+; and (k) w1118; ptc-Gal4, UAS-dE2f1dsRNA#3/+; His2AvD05146/+. At least 15 to 25 wings of each genotype (a∼k) were measured. Each genotype (c∼k) was compared with the control (b: w1118; ptc-Gal4, UAS-dE2f1dsRNA#3/+; +) and each comparison is highly significant (P < 4.9E-06 based on one-tailed t-test). For simplicity, “ptc-Gal4, UAS-dE2f1dsRNA#3” is referred as “PE3” in (E). The scale bar in (D) is 100µm.