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. 2012 Dec 1;2(12):1687–1701. doi: 10.1534/g3.112.003814

Figure 3 .

Figure 3 

Extragenic ipl1-2 suppressors in SDS22. (A) Cultures of WT (KT1113), ipl1-2 (KT1829), ipl1-2 sds22-D2N D119N (KT2934), ipl1-2 sds22-F177S (KT3353), ipl1-2 sds22-W187R (KT3381), ipl1-2 sds22-E163I L329P (KT3052), and ipl1-2 sds22-77 (KT2936) strains were serially diluted onto YPD medium and imaged after 40 hr at the designated temperatures. (B) ClustalW alignment of human and S. cerevisiae Sds22 proteins showing locations of ipl1 suppressor mutations. Boxed residues correspond to sites of mutations in human Sds22 that prevent proper binding of PP1 (Ceulemans et al. 2002). (C) Cultures of WT (KT1113), ipl1-2 (KT1829), sds22-D2N D119N (KT2963), ipl1-2 sds22-D2N D119N (KT2934), sds22-F177S (KT3351), ipl1-2 F177S (KT3353), sds22-E163I L329P (KT3292), ipl1-2 sds22-E163I L329P (KT3052), sds22-77 (KT2964), and ipl1-2 sds22-77 (KT2936) strains were serially diluted onto YPD medium and YPD medium containing 3% formamide and imaged after 24 hr and 72hr at 24°, respectively. (D) Cultures of WT (KT1113), sds22-D2N D119N (KT2963), sds22-F177S (KT3351), sds22-E163I L329P (KT3292), and sds22-77 (KT2964) strains were serially diluted onto the designated medium at the designated temperatures. Media, with the exception of SC, are either YPD or YPD supplemented as indicated. The YPD 14° plates were incubated for 14 d. All other plates were incubated for 40 hr.