Table 1. Summary of ipl1-2 suppressor mutations.
| Suppressor Locus Mutant Allele | Revertant No. | Mutation (S) | Residue in Human Ortholog |
|---|---|---|---|
| IPL1 | |||
| IPL1-R150K H352Y | 14,21 | G449A, C1054T | R139 H340 |
| IPL1-S167L H352Y | 16 | C500T, C1054T | M136 H340 |
| IPL1-G347E H352Y | 20 | G1040A, C1054T | K335 H340 |
| GLC7 (Type 1 protein phosphatase [PP1] catalytic subunit) | |||
| glc7-L15S | 106 | T44C | L16 |
| glc7-L37F | 85 | A111T, T175A | L38 |
| glc7-L71S | 82 | T737C | L72 |
| glc7-L74P | 39, 62, 89 | T746C | L75 |
| glc7-Y92N R141K | 55 | T799A, G947A | Y93 R142 |
| glc7-S99L | 84 | T820C, C821T | S100 |
| glc7-K112E | 107 | A859G | K113 |
| glc7-F118S | 102 | T878C | F119 |
| glc7-Y136N | 73 | T931A | Y137 |
| glc7-Q293D | 112 | A1403C | Q294 |
| YPI1 (Glc7/PP1 regulatory subunit) | |||
| ypi1-F74S | 52 | T221C | Y64 |
| ypi1-F74L | 95 | T222A | Y64 |
| SDS22 (Glc7/PP1 regulatory subunit) | |||
| sds22-F177S | 47 | T530C | I204 |
| sds22-W187R | 53 | T559A | F214 |
| sds22-D2N D119N | 74 | G4A, G355A | D148 |
| sds22-77 (C-terminal frameshift)a | 77 | ΔC1014 | |
| sds22-E163I L329P | 94 | 486AGA488GAT, T984C | E192 V350 |
| SHP1 (subunit of Cdc48/p97 AAA AATPase) | |||
| shp1-FS99b | 99 | A insertion after 368, T370C | |
| shp1-FS105c | 105 | A insertion after 873, G877A | |
| TCO89 (nonessential subunit of TOR Complex 1) | |||
| tco89-71 | 71 | four missense codons and a stop codond | not conserved |
| DUO1 (subunit of DAM/DASH complex) | |||
| duo1-S115F | 76, 81 | C344T | not conserved |
| NDC80 (subunit of Ndc80 complex) | |||
| ndc80-K204E | 8 | A610G | K166 |
a
Frameshift at final codon (G 338 underlined) resulting in stop after 22 missense codons: …YIRG* →…YIRGDLDQEMIFFLYTHTNIYIYIYI.*
b
Frameshift after codon 122 (underlined) resulting in stop after 15 missense codons: …GSGNN… →…GSGNKPQVYELFGYGKRSS*
c
Frameshift after codon 291 (underlined) resulting in stop after 2 missense codons: …NVYKKLD… →…NVYKKIK*