Table II.

Characteristics of some T₂ progeny obtained by selfing transformants with elevated and reduced levels of leaf catalase specific activities

Plant No. Selfed	Plasmid	Kan-Resistant Seedling Growth		Progeny with CAT Specific Activity Different from WT		Mean Change in CAT Specific Activity
		n tested	% resistant	n assayed	% altered CAT	Ratio transgenic:WT
2.1E	pBZ1	19	63	12	17	0.60  ± 0.07
2.2Aa	pBZ1	21	57	23	17	0.44  ± 0.06
2.3B	pBZ1	35	49	10	20	0.43  ± 0.06
4.7Da	pBZ1	61	89	54	11	0.62  ± 0.09
7.2.3	pBZ6	98	57	15	20	0.61  ± 0.09
10.33b	pBZ2b	20	100	13	8	0.52  ± 0.07
9.8	pBZ8	79	41	28	32	1.74  ± 0.13
9.11	pBZ8	68	59	12	25	1.76  ± 0.11
6.19.1	pAC1352L	60	93
WT		219	4

The plasmids (Table I) are defined in Methods. Kanamycin (Kan)-resistant growth of seedlings was determined as described in the text. The number of seedlings examined for Kan-resistant growth (75 or 100 μg/mL) in each family, the number of randomly selected plants (without testing for Kan resistance) assayed for leaf catalase specific activity in each family, and the percentage that clearly differed from WT (± sd) of the means are shown.

^aThe presence of NPTII was confirmed by Southern-blot analysis on all selfed progeny of plant 2.2A and plant 4.7D, which showed alteration from WT in leaf catalase specific activity, although siblings with WT catalase activity also frequently gave positive signals. 

^bThe parent plant 10.33 had decreased catalase activity presumably because of co-suppression (Table I).